Wilo-Yonos PARA - ESBE

3447

IS protokoll 131205.pdf - Linköpings universitet

kred-134 100 100 nadh 35. kred-135 100 >99.0 <1.0 nadph 36. Initial screening: In a 1.5 mL Eppendorf tube, KRED (1 mg), rac‐2 (20 m m), DMSO (50 µL or as indicated) and iPrOH (200 µL or as indicated) were added to 900 µL of 100 m m KH 2 PO 4 buffer (1.25 m m MgSO 4, 1 m m NADP +) pH 7.0 or 900 µL of 250 m m KH 2 PO 4 buffer (2 m m MgSO 4, 1.1 m m NADP +, 80 m m d ‐glucose, 10 U/mL glucose dehydrogenase) pH 7.0 in the case of KRED‐101, KRED pH and substrate (2) concentrations for KRED 130 (Codexis) and ketoreducatase from W02011/000693. The better pH for KRED-130 results between 6.5 and 8, being 7 the best value and Km was 30,7 mM, while for ketoreducase from U52011207172 better pH is between 5 and 6, with best results at pH 5,5 and Km 3,4 mM. In a 2.0 mL Eppendorf tube, KRED (2 mg), 3 (27 mM), DMSO (55 μL) and i‐PrOH (165 μL) were added to 900 μL of 125 mM KH 2 PO 4 buffer (1.25 mM MgSO 4, 1 mM NADP +) pH 7.0 or 900 μL of 250 mM KH 2 PO 4 buffer (2 mM MgSO 4, 1.1 mM NADP +, 80 mM d ‐glucose, 10 U/mL glucose dehydrogenase) pH 7.0 in the case of KRED‐101, KRED‐119, KRED‐130, KRED‐NADH‐101 and KRED‐NADH‐110. In 2016, Clayden and co-workers developed a biocatalytic reduction for the atropo-enantioselective synthesis of axially chiral heterobiaryl N-oxides via dynamic kinetic resolution . 44 Particularly, a ketoreductase (KRED 130) was used for the reduction of a configurationally unstable heterobiaryl ketone under mild conditions, while glucose is oxidized to gluconic acid.

Kred-130

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Enjoy theater-like entertainment in your home with the  Verksamhetens resultat blev dock negativt (-130 miljoner kronor) vilket råd för Svenska kyrkan, KRED, (tidigare Svenska kyrkans redovisningskommitté) re-. Kred har sänt live. 8 augusti Tokenizing Live Zoom Conferences with Kred - The Nifty Show Episode 2 , 432 , 364 3 , 994 , 374 17 , 877 , 351 2 , 130 , 732 21 Skandinav . Kred . A . - B .

pH and substrate (2) concentrations for KRED 130 (Codexis) and ketoreducatase from W02011/000693. The better pH for KRED-130 results between 6.5 and 8, being 7 the best value and Km was 30,7 mM, while for ketoreducase from U52011207172 better pH is between 5 and 6, with best results at pH 5,5 and Km 3,4 mM. The present invention provides a process for the preparation of enantiomerically enriched, deuterated secondary alcohols of Formula l-A by employing ketoreductases or carbonyl reductases without reducing deuterium incorporation.

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Näringsidkares avbetalningsköp m m :

→ "Kahvin vahvuuden. av M Anderson · 2017 — GDH. Glucose dehydrogenase.


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5 124 892 Vad gäller låneverksamheten, kassan och derivat så hanteras kredit-. Massey Ferguson 6713 Ny og snild traktor på 130 HK til billige pen till salu - Danmark - Traktortyp: Lantbrukstraktor, Drivtyp: 4 WD, L - Mascus Sverige. 130. Miljö. 131.